Screening of orange peel waste on valuable compounds by gradient multiple development diode-array high-performance thin-layer chromatography

Abstract High-performance thin-layer chromatography (HPTLC), as the modern form of TLC (thin-layer chromatography), is suitable for detecting pharmaceutically active compounds over a wide polarity range using gradient multiple development (GMD) technique. Diode-array detection (DAD) in conjunction with HPTLC can simultaneously acquire ultraviolet?visible (UV?VIS) and fluorescence spectra directly from plate. Visualization contour plot helps to identify separated zones. An orange peel extract used an example show how GMD?DAD?HPTLC seven different developments solvents provide overview entire sample. More than 50 be on 6-cm Such separations take place biologically inert stationary phase HPTLC, making it method effect-directed analysis (EDA). HPTLC?EDA even performed living organism, confirmed by use Aliivibrio fischeri bacteria detect bioluminescence measure toxicity. The combining planar diode-array (GMD?DAD?HPTLC?EDA) specific staining methods time-of-flight mass spectrometry (TOF?MS) will choice find new chemical structures plant extracts that serve basic structure compounds.